Anticorps Monoclonal anti-CCDC6

CCDC6 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1D6A8

N° de cat : 67637-1-Ig

Synonymes

CCDC6, D10S170, FLJ32286, H4, Protein H4, PTC, TPC, TST1

---

Galerie de données de validation

• 

  WB analysis using 67637-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67637-1-Ig (CCDC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 67637-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67637-1-Ig (CCDC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of U2OS using 67637-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67637-1-Ig (CCDC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 67637-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CCDC6 antibody (67637-1-Ig, Clone: 1D6A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A549, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67637-1-Ig cible CCDC6 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCDC6 Protéine recombinante Ag6952
• Nom complet: coiled-coil domain containing 6
• Masse moléculaire calculée: 474 aa, 53 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC036757
• Symbole du gène: CCDC6
• Identification du gène (NCBI): 8030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCDC6 (Coiled-coil domain-containing protein 6) is also named as Protein H4, D10S170 and TST1. CCDC6, was initially isolated as part of a tumorigenic DNA originated by the fusion of CCDC6 with the tyrosine kinase of RET receptor. CCDC6 has been considered as an accidental partner of the RET protooncogene, providing the promoter and the first 101 aa necessary for the constitutive activation of the oncogenic Tyrosine Kinase (TK) RET in thyroid cells. The 65 kDa product of CCDC6 has a nuclear transfer sequence with no transmembrane domains and is predicted to locate in both the nucleus and the cytoplasm (PMID: 29044514). The CCDC6 is a phosphoprotein, predicted target of several S/T kinases which can modulate the protein stability and the intracellular shuttling into the nucleus upon different cellular signals mediated by ERK1/2, ATM and CDK1/2. CCDC6 is involved in cellular response to DNA damage mediated by ATM, with the final result of promoting cellular apoptosis. CCDC6 depleted cells are considered defective of DNA repair checkpoint and proceed faster than the control cells in the cell cycle upon induced DNA damage (PMID: 22655027).