Anticorps Monoclonal anti-CCAR2

• Phare
• Validé par KD/KO

CCAR2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

2D10F8

N° de cat : 66497-1-Ig

Synonymes

CCAR2, cell cycle and apoptosis regulator 2, DBC 1, DBC.1, DBC1, KIAA1967, p30 DBC, Protein KIAA1967

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Galerie de données de validation

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  WB analysis using 66497-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66497-1-Ig

  WB result of CCAR2 antibody (66497-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCAR2 transfected HEK-293 cells.

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  WB analysis of pig brain using 66497-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human colon using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human lung cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66497-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66497-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66497-1-Ig (CCAR2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of U2OS using 66497-1-Ig

  1X10^6 U2OS cells were intracellularly stained with 0.2 ug Anti-Human CCAR2 (66497-1-Ig, Clone:2D10F8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules MCF-7, tissu cérébral de porc
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66497-1-Ig cible CCAR2 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CCAR2 Protéine recombinante Ag18374
• Nom complet: KIAA1967
• Masse moléculaire calculée: 923 aa, 103 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC018269
• Symbole du gène: KIAA1967
• Identification du gène (NCBI): 57805
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).