Anticorps Monoclonal anti-CBX3

CBX3 Monoclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1A5A3

Avis (1)

N° de cat : 66446-1-Ig

Synonymes

CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 66446-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66446-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66446-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66446-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66446-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 66446-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66446-1-Ig (CBX3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66446-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66446-1-Ig (CBX3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules RAW 264.7
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66446-1-Ig cible CBX3 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CBX3 Protéine recombinante Ag19112
• Nom complet: chromobox homolog 3 (HP1 gamma homolog, Drosophila)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC000954
• Symbole du gène: CBX3
• Identification du gène (NCBI): 11335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins

Publications

Species	Application	Title
human	WB	Int J Biochem Cell Biol; NPM1 promotes cell proliferation by targeting PRDX6 in colorectal cancer.; Authors - Dan Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

ILIAS (Verified Customer) (05-26-2023)

The 66446-1-Ig worked very well in both Western Blot and Immunofluorescence microscopy experiments

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 (WB), 1:500(IF)
• Cell Tissue Type: HeLa and MCF7 cells

CBX3 Antibody Western Blot,Immunofluorescence validation (1:2000 (WB), 1:500(IF) dilution) in HeLa and MCF7 cells (Cat no:66446-1-Ig)