Anticorps Monoclonal anti-CBFB

CBFB Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

1D7F2

N° de cat : 67885-1-Ig

Synonymes

CBF beta, CBFB, PEA2 beta, PEBP2 beta, PEBP2B

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Galerie de données de validation

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  WB analysis using 67885-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HEK-293 using 67885-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67885-1-Ig (CBFB antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 67885-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CBFB (67885-1-Ig, Clone:1D7F2) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of K-562 using 67885-1-Ig

  1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CBFB (67885-1-Ig, Clone:1D7F2) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HSC-T6, cellules K-562, cellules MOLT-4
• Résultats positifs en cytométrie: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67885-1-Ig cible CBFB dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CBFB Protéine recombinante Ag31183
• Nom complet: core-binding factor, beta subunit
• Masse moléculaire calculée: 182 aa, 22 kDa
• Numéro d’acquisition GenBank: BC018509
• Symbole du gène: CBFB
• Identification du gène (NCBI): 865
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CBFB (core binding factor subunit β), forms the heterodimeric complex core-binding factor (CBF). As a critical transcription factor, CBFB is frequently mutated in breast cancer and considered to be of significance in the pathogenesis of cancer.(PMID: 29932212) The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters.) It has calculated molecular weight around 22kDa.