Anticorps Monoclonal anti-Caspase 3/p17/p19

• Phare
• Validé par KD/KO

Caspase 3/p17/p19 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (5)

Applications

WB, IHC, IF, ICC, ELISA

Conjugaison

Non conjugué

Publications

214

CloneNo.

2G4B2

Avis (1)

N° de cat : 66470-2-Ig

Synonymes

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17/p19, Caspase3, Caspase-3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

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Galerie de données de validation

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  WB analysis of HEK-293 using 66470-2-Ig

  WB result of Caspase 3 antibody (66470-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected HEK-293 cells.

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  WB analysis of Jurkat using 66470-2-Ig

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66470-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66470-2-Ig (Caspase 3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66470-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66470-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66470-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66470-2-Ig

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66470-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66470-2-Ig (CASP3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66470-2-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 3/p17/p19 antibody (66470-2-Ig, Clone: 2G4B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HepG2, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu hépatique de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 196 publications below
• IHC: See 18 publications below
• IF: See 13 publications below

Informations sur le produit

66470-2-Ig cible Caspase 3/p17/p19 dans les applications de WB, IHC, IF, ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, canin, Humain, plante, porc, poulet, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Caspase 3/p17/p19 Protéine recombinante Ag25029
• Nom complet: caspase 3, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 277 aa, 32 kDa
• Poids moléculaire observé: 32-35 kDa, 19 kDa, 17 kDa
• Numéro d’acquisition GenBank: BC016926
• Symbole du gène: CASP3
• Identification du gène (NCBI): 836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

Publications

Species	Application	Title
rat	WB,ICC	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu
human	WB	Small; Inherent Capability of Self-Assembling Nanostructures in Specific Proteasome Activation for Cancer Cell Pyroptosis; Authors - Qian-Wei Luo
mouse	WB	Nat Commun; BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency; Authors - Feixia Wang
rat	WB	Acta Biomater; Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.; Authors - Shanshan Ma
human	WB	Biomaterials; Autophagy-inhibiting polymer as an effective nonviral cancer gene therapy vector with inherent apoptosis-sensitizing ability.; Authors - Jiafeng Wang
human	WB	ACS Appl Mater Interfaces; Endoplasmic Reticulum-Targeted Aggregation-Induced Emission Luminogen for Synergetic Tumor Ablation with Glibenclamide; Authors - Yifan Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

K. (Verified Customer) (10-26-2023)

This Ab is working good for human, rat and mouse proteins samples.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Liver cells