- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-CARM1
CARM1 Polyclonal Antibody for IF, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, singe, souris
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugaison
Non conjugué
N° de cat : 55246-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en IHC | tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain, tissu de mélanome malin humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF | cellules NIH/3T3 |
Dilution recommandée
Application | Dilution |
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Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 3 publications below |
WB | See 8 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Informations sur le produit
55246-1-AP cible CARM1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris
Réactivité | Humain, rat, singe, souris |
Réactivité citée | Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Peptide |
Nom complet | coactivator-associated arginine methyltransferase 1 |
Masse moléculaire calculée | 66 kDa |
Poids moléculaire observé | 63-66 kDa, 55 kDa |
Numéro d’acquisition GenBank | NM_199141 |
Symbole du gène | CARM1 |
Identification du gène (NCBI) | 10498 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), β-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated through phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTM sites in cells themselves.
Protocole
Product Specific Protocols | |
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WB protocol for CARM1 antibody 55246-1-AP | Download protocol |
IHC protocol for CARM1 antibody 55246-1-AP | Download protocol |
IF protocol for CARM1 antibody 55246-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Med Chem Discovery of First-in-Class Dual PARP and EZH2 Inhibitors for Triple-Negative Breast Cancer with Wild-Type BRCA | ||
Eur J Med Chem Discovery of precision targeting EZH2 degraders for triple-negative breast cancer. | ||
Front Cell Dev Biol Osteocytes Enhance Osteogenesis by Autophagy-Mediated FGF23 Secretion Under Mechanical Tension. | ||
Cancer Cell Int CDCA4 suppresses epithelial-mesenchymal transtion (EMT) and metastasis in Non-small cell lung cancer through modulating autophagy.
| ||
RSC Adv Mechanistic insights into geniposide regulation of bile salt export pump (BSEP) expression | ||
J Reprod Immunol Poly(I:C) exposure during in vitro fertilization disrupts first cleavage of mouse embryos and subsequent blastocyst development. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH fatemeh (Verified Customer) (07-25-2022) | western blot was done on 50 ug of cell lysate. Blot was transfered using wet transfer method (300 mA for 90 min). 5% milk was used as blocking reagent. antibody dilution was 1:1000 and incubation with primary antibody was overnight at 4C. Multiple band between 75 and 50 kDa were detected. for IF, PFA was used for fixing the cells and 0.5% triton was used for permeabilization. antibody was diluted 1:200 and samples were incubated with primary ab for 1h at RT. a cytoplasmic signal for Carm1 was detected.
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FH Kyosuke (Verified Customer) (06-12-2019) | It works well on HUVECs for Western blot.
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