Anticorps Monoclonal anti-CAPNS1

CAPNS1 Monoclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

CloneNo.

2B6G6

N° de cat : 67838-1-Ig

Synonymes

30K, Calpain regulatory subunit, Calpain small subunit 1, calpain, small subunit 1, CALPAIN4, CANP, CANP small subunit, CANPS, CAPN4, CAPNS, CAPNS1, CDPS, CSS1

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Galerie de données de validation

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  WB analysis of A375 using 67838-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67838-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67838-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67838-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67838-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 67838-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 67838-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67838-1-Ig (CAPNS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 67838-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CAPNS1 antibody (67838-1-Ig, Clone: 2B6G6 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules A431, cellules HeLa, cellules HSC-T6, cellules K-562, cellules PC-12, cellules THP-1
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67838-1-Ig cible CAPNS1 dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CAPNS1 Protéine recombinante Ag21885
• Nom complet: calpain, small subunit 1
• Masse moléculaire calculée: 28 kDa
• Numéro d’acquisition GenBank: BC011903
• Symbole du gène: CAPNS1
• Identification du gène (NCBI): 826
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.