Anticorps Monoclonal anti-CAP2

CAP2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1G7D2

N° de cat : 67412-1-Ig

Synonymes

CAP 2, CAP2

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Galerie de données de validation

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  WB analysis using 67412-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of HepG2 using 67412-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 67412-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 67412-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67412-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67412-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67412-1-Ig (CAP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules COLO 320, cellules HeLa, cellules HepG2, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67412-1-Ig cible CAP2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CAP2 Protéine recombinante Ag8622
• Nom complet: CAP, adenylate cyclase-associated protein, 2 (yeast)
• Masse moléculaire calculée: 477 aa, 53 kDa
• Poids moléculaire observé: 51-53 kDa
• Numéro d’acquisition GenBank: BC008481
• Symbole du gène: CAP2
• Identification du gène (NCBI): 10486
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CAPs (CAP1 and CAP2) are evolutionarily conserved proteins with roles in regulating the actin cytoskeleton and in signal transduction. CAP2 is predominantly expressed in brain, heart and skeletal muscle, and skin. It is found in the nucleus in undifferentiated myoblasts and at the M-line of differentiated myotubes. Overexpression of CAP2 has been reported in many cancers, including hepatocellular carcinoma (HCC), human breast cancer, and malignant melanoma.