Anticorps Polyclonal de lapin anti-CACNA2D1

CACNA2D1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 27453-1-AP

Synonymes

CACNA2, CACNA2D1, CACNL2A, CCHL2A, MHS3

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Galerie de données de validation

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  WB analysis of mouse brain using 27453-1-AP

  mouse brain tissue and mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 27453-1-AP (CACNA2D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 27453-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 27453-1-AP (CACNA2D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 27453-1-AP

  pig brain tissue were subjected to SDS PAGE followed by western blot with 27453-1-AP (CACNA2D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 27453-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 27453-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 27453-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 27453-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse eye using 27453-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CACNA2D1 antibody (27453-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 27453-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CACNA2D1 antibody (27453-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de porc, tissu cérébral de rat, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu oculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 4 publications below

Informations sur le produit

27453-1-AP cible CACNA2D1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CACNA2D1 Protéine recombinante Ag26190
• Nom complet: calcium channel, voltage-dependent, alpha 2/delta subunit 1
• Masse moléculaire calculée: 125 kDa
• Poids moléculaire observé: 125 kDa
• Numéro d’acquisition GenBank: NM_000722
• Symbole du gène: CACNA2D1
• Identification du gène (NCBI): 781
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	IF,WB	Brain Behav Immun; Microglial IL-10 and β-endorphin expression mediates gabapentinoids antineuropathic pain.; Authors - Khalil Ali Ahmad
rat	WB,IF	Heliyon; Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1; Authors - Chuan Jiang
human	WB,IF	Cells; Calcium-Related Genes Predicting Outcomes and Serving as Therapeutic Targets in Endometrial Cancer; Authors - Ting Huang
mouse	WB,IF	Ecotoxicol Environ Saf; Cigarette smoke triggers calcium overload in mouse hippocampal neurons via the ΔFOSB-CACNA2D1 axis to impair cognitive performance; Authors - Hongchao Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christian (Verified Customer) (11-19-2018)

1:50 - labelled neurons of interest1:200 - labelled 3-4 neurons of interest1:1000 - labelled 1-2 neurons of interest

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:50, 1:200, 1:1000
• Cell Tissue Type: Mouse Spinal Cord/Neurons

CACNA2D1 Antibody Immunohistochemistry,Immunofluorescence, validation (1:50, 1:200, 1:1000 dilution) in Mouse Spinal Cord/Neurons (Cat no:27453-1-AP)