Anticorps Polyclonal de lapin anti-cGAS

• Phare
• Validé par KD/KO

cGAS Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

63

Avis (4)

N° de cat : 26416-1-AP

Synonymes

C6orf150, cGAS, Cyclic GMP-AMP synthase, h-cGAS, MB21D1

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Galerie de données de validation

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  WB analysis using 26416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26416-1-AP (cGAS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26416-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26416-1-AP (cGAS antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 26416-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using cGAS antibody (26416-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 48 publications below
• IHC: See 9 publications below
• IF: See 22 publications below
• IP: See 1 publications below

Informations sur le produit

26416-1-AP cible cGAS dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: cGAS Protéine recombinante Ag23455
• Nom complet: chromosome 6 open reading frame 150
• Masse moléculaire calculée: 496 aa, 54 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC113608
• Symbole du gène: C6orf150
• Identification du gène (NCBI): 115004
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
human	WB,IF	Cell; Poxviruses Evade Cytosolic Sensing through Disruption of an mTORC1-mTORC2 Regulatory Circuit.; Authors - Nathan Meade
	WB,IF	Adv Sci (Weinh); PICH Supports Embryonic Hematopoiesis by Suppressing a cGAS-STING-Mediated Interferon Response.; Authors - Xinwei Geng
human	WB	Nat Commun; ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling; Authors - Maximilian Hirschenberger
	WB	Mol Cell; CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity; Authors - Guiheng Zhang ; KO Validated
human	WB	J Clin Invest; Melatonin inhibits cytosolic mitochondrial DNA-induced neuroinflammatory signaling in accelerated aging and neurodegeneration.; Authors - Abhishek Jauhari

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sonia (Verified Customer) (09-12-2023)

Very clear staining of ruptured micronuclei in U2OS cells, fixed in 4% FA and permeabilised with PBS-Triton 0.5%. Primary antibody incubation was performed o/n at 4ºC in blocking buffer at 1:250.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: U2OS cells

FH

Shunbin (Verified Customer) (06-15-2023)

Produces a specific band and two other non-specific bands under the indicated conditions. The other cGAS Ab from Cell Signaling Technology produces one single band. (compare images attached

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

cGAS Antibody Western Blot validation (1:1000 dilution) in (Cat no:26416-1-AP)

FH

Zhiping (Verified Customer) (03-18-2022)

Good Ab for WB

• Applications: Western Blot

FH

Coralie (Verified Customer) (10-20-2021)

Cytosolic staining of cGAS (forms dots, after PFA fixation).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: HFF

cGAS Antibody Immunofluorescence validation (1/100 dilution) in HFF (Cat no:26416-1-AP)