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Anticorps Monoclonal anti-Beta Actin

Beta Actin Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

chien, Humain, poisson-zèbre, rat, singe, souris, Hamster

Applications

IF, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

2D4H5

N° de cat : CL555-66009

Synonymes

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cytoskeleton Marker, F actin, PS1TP5BP1, β actin

Galerie de données de validation

Filter:
  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite®555-conjugated Beta Actin Monoclonal anti (CL555-66009)

    IF Staining of A549 using CL555-66009

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®555 Beta Actin antibody (CL555-66009, Clone: 2D4H5 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated Beta Actin Monoclonal anti (CL555-66009)

    FC experiment of HeLa using CL555-66009

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Beta Actin (CL555-66009, Clone:2D4H5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules A549,
Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-66009 cible Beta Actin dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons chien, Humain, poisson-zèbre, rat, singe, souris, Hamster

Réactivité chien, Humain, poisson-zèbre, rat, singe, souris, Hamster
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène Protéine recombinante
Nom complet actin, beta
Masse moléculaire calculée 42 kDa
Poids moléculaire observé 42 kDa
Numéro d’acquisition GenBankNM_001101
Symbole du gène ACTB
Identification du gène (NCBI) 60
Conjugaison CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates. Beta-actin (or β-actin) is a cytoskeletal actin isoform, highly conserved and ubiquitously expressed in eukaryotic organisms, making it a commonly used "housekeeping gene" in many laboratory techniques. It has been widely used as the internal control in RT-PCR and Western Blotting. The isotype of this antibody is IgG2b.

 

What is the molecular weight of beta-actin?

42 kDa

 

What is the function of beta-actin?

Actins are a key part of the cytoskeleton and are involved in cell motility, structure, and signaling, with beta-actin as part of the contractile apparatus. Beta-actin is located in the cytoplasm of non-muscle cells, particularly where the plasma membrane meets the cytoskeleton (PMID: 1993736) and is found co-distributed with alpha-actin (PMID: 9490838).

 

What are the applications for beta-actin?

As it is ubiquitously and constitutively expressed, beta-actin is a useful loading control in techniques such as RT-PCR and Western Blots to measure baseline expression and degradation. Beta-actin can be used as a reference gene to normalize mRNA or protein levels in quantitative studies of expression. Although some evidence suggests variation in tissue types and developing embryos (PMID: 23099774) if expression is stable across conditions, beta-actin is still considered by many to be a suitable gene for normalization.

Beyond its role as a housekeeping gene, it has been shown that beta-actin associates with endothelial nitric-oxide synthase (eNOS) and can therefore regulate nitric oxide activity in endothelial cells (PMID: 12734108).

 

What are the post-transcriptional modifications (PTMs) to beta actin?

Alterations to actin proteins can modulate their properties and function. While small molecules are known to alter actin, post-translational modifications such as phosphorylation and acetylation can also affect actin (PMID: 23195437). Modifications to actin have been associated with diseases including but not limited to age-related macular degeneration (PMID: 17652714), cardiovascular disease (PMID: 21232667), and Alzheimer's disease (PMID: 11246152). 


For murine tissue sample, conjugated antibody (HRP-66009) or rabbit antibody (20536-1-AP) is preferable.



Protocole

Product Specific Protocols
IF protocol for CL555 Beta Actin antibody CL555-66009Download protocol
Standard Protocols
Click here to view our Standard Protocols