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Anticorps Monoclonal anti-Bcl2

Bcl2 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

1B5B9

N° de cat : CL555-66799

Synonymes

AW986256, B cell leukemia/lymphoma 2, Bcl2, C430015F12Rik, D630044D05Rik, D830018M01Rik

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CoraLite®555-conjugated Bcl2 Monoclonal antibody (CL555-66799)

    IF Staining of NIH/3T3 using CL555-66799

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite®555 Bcl2 antibody (CL555-66799, Clone: 1B5B9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite®555-conjugated Bcl2 Monoclonal antibody (CL555-66799)

    FC experiment of NIH/3T3 using CL555-66799

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Mouse Bcl2 (CL555-66799, Clone:1B5B9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules NIH/3T3,
Résultats positifs en cytométriecellules NIH/3T3

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-66799 cible Bcl2 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Bcl2 Protéine recombinante Ag24261
Nom complet B-cell leukemia/lymphoma 2
Masse moléculaire calculée 26 kDa
Poids moléculaire observé 26 kDa
Numéro d’acquisition GenBankNM-009741
Symbole du gène Bcl2
Identification du gène (NCBI) 12043
Conjugaison CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

Protocole

Product Specific Protocols
IF protocol for CL555 Bcl2 antibody CL555-66799Download protocol
FC protocol for CL555 Bcl2 antibody CL555-66799Download protocol
Standard Protocols
Click here to view our Standard Protocols