Anticorps Polyclonal de lapin anti-Bcl-XL

Bcl-XL Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

66

N° de cat : 26967-1-AP

Synonymes

Apoptosis regulator Bcl X, BCL2L1, Bcl-xL

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 26967-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26967-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 26967-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26967-1-AP (Bcl-XL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 26967-1-AP

  IP result of anti-Bcl-XL (IP:26967-1-AP, 4ug; Detection:26967-1-AP 1:1000) with NIH/3T3 cells lysate 2000 ug.

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  IHC staining of human testis using 26967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26967-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 26967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26967-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 26967-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26967-1-AP (Bcl-XL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26967-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26967-1-AP (Bcl-XL/S antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using 26967-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human Bcl-XL (26967-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IP: cellules NIH/3T3
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 65 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

26967-1-AP cible Bcl-XL dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Bcl-XL Protéine recombinante Ag25604
• Nom complet: BCL2-like 1
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC019307
• Symbole du gène: BCL2L1
• Identification du gène (NCBI): 598
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. This antibody can recognize Bcl-XL, Bcl-XS and Bcl-X(Beta).

Publications

Species	Application	Title
rat	WB	Microbiome; The microbiota-gut-brain axis participates in chronic cerebral hypoperfusion by disrupting the metabolism of short-chain fatty acids.; Authors - Weiping Xiao
human	WB	Clin Cancer Res; Long noncoding RNA LINC01234 functions as a competing endogenous RNA to regulate CBFB expression by sponging miR-204-5p in gastric cancer.; Authors - Xin Chen
human	WB	Clin Cancer Res; Inhibition of Bcl-2 Synergistically Enhances the Antileukemic Activity of Midostaurin and Gilteritinib in Preclinical Models of FLT3-mutated Acute Myeloid Leukemia.; Authors - Jun Ma
human	WB	Cell Rep; Developmental potency of human ES cell-derived mesenchymal stem cells revealed in mouse embryos following blastocyst injection; Authors - Borong Huang
mouse	WB	Int J Biol Macromol; CircDYM attenuates microglial apoptosis via CEBPB/ZC3H4 axis in LPS-induced mouse model of depression; Authors - Zhongqiu Zhou
human	WB,IHC	Cancer Lett; Esculetin inhibits endometrial cancer proliferation and promotes apoptosis via hnRNPA1 to downregulate BCLXL and XIAP.; Authors - Ruqi Jiang