Anticorps Monoclonal anti-BSA

BSA Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

bovin et plus (3)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

13

CloneNo.

2D11G3

Avis (2)

N° de cat : 66201-1-Ig

Synonymes

Bos d 6, BSA, Serum albumin

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Galerie de données de validation

• 

  WB analysis using 66201-1-Ig

  20ng, 10ng and 5ng BSA were subjected to SDS PAGE followed by western blot with 66201-1-Ig (BSA Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of bovine serum using 66201-1-Ig

  Bovine serum were subjected to SDS PAGE followed by western blot with 66201-1-Ig (BSA Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: bsa, tissu sérique bovin

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below

Informations sur le produit

66201-1-Ig cible BSA dans les applications de WB, ELISA et montre une réactivité avec des échantillons bovin

• Réactivité: bovin
• Réactivité citée: Humain, souris, Pcn033
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine naturelle
• Nom complet: bovine serum albumin
• Poids moléculaire observé: 67 kDa
• Symbole du gène: BSA
• Identification du gène (NCBI): 280717
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BSA, also named as ALB and Bovine Serum albumin, is the main protein of plasma. It has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood.

Publications

Species	Application	Title
	WB	Cell Metab; Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.; Authors - Ke Wang
	WB	Redox Biol; Small extracellular vesicles from senescent stem cells trigger adaptive mechanisms in young stem cells by increasing antioxidant enzyme expression; Authors - Cristina Mas-Bargues
	WB	Dev Cell; Transnitrosylation Mediated by the Non-canonical Catalase ROG1 Regulates Nitric Oxide Signaling in Plants.; Authors - Lichao Chen
human	WB	Int J Nanomedicine; Exosomal Vaccine Loading T Cell Epitope Peptides of SARS-CoV-2 Induces Robust CD8+ T Cell Response in HLA-A Transgenic Mice; Authors - An-Ran Shen
human	WB	J Nanobiotechnology; Protective role of small extracellular vesicles derived from HUVECs treated with AGEs in diabetic vascular calcification; Authors - Bei Guo
mouse	WB	J Virol; Amino acid substitutions in norovirus VP1 dictate host dissemination via variations in cellular attachment; Authors - Jake T Mills

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ning (Verified Customer) (02-27-2023)

Can perfectly recognize the internalized BSA-conjugated probe.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: primary mouse neurons

FH

Jake (Verified Customer) (08-25-2021)

Overall the antibody worked great. It was clean, detected the correct band and worked with my samples. Very Happy with product

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: BV-2 Cells (supernatant)