Anticorps Polyclonal de lapin anti-BRD7

• Phare
• Validé par KD/KO

BRD7 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 51009-2-AP

Synonymes

75 kDa bromodomain protein, BP75, BRD7, bromodomain containing 7, CELTIX1, NAG4, Protein CELTIX 1

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Galerie de données de validation

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  WB analysis of HeLa using 51009-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51009-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51009-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 51009-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 51009-2-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 51009-2-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 51009-2-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 51009-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 51009-2-AP (BRD7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 51009-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51009-2-AP (BRD7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 51009-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51009-2-AP (BRD7 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 51009-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51009-2-AP (BRD7 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules Jurkat, cellules L02, cellules MDA-MB-231, cellules PC-3, cellules SH-SY5Y, tissu cérébral de rat, tissu de cervelet de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 23 publications below
• IHC: See 9 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

51009-2-AP cible BRD7 dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BRD7 Protéine recombinante Ag3911
• Nom complet: bromodomain containing 7
• Masse moléculaire calculée: 651 aa, 74 kDa
• Poids moléculaire observé: 70-74 kDa
• Numéro d’acquisition GenBank: BC050728
• Symbole du gène: BRD7
• Identification du gène (NCBI): 29117
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.

Publications

Species	Application	Title
human	WB	Cancer Discov; A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction.; Authors - Jinxiu Li
mouse	IF	Nat Commun; SS18 regulates pluripotent-somatic transition through phase separation.; Authors - Junqi Kuang
human	WB	Cell Rep; FAK-mediated phosphorylation at Y464 regulates p85β nuclear translocation to promote tumorigenesis of ccRCC by repressing RB1 expression; Authors - Yanhua Zhang
human	WB,CoIP	Cancer Res; BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription.; Authors - Harte Mary T MT ; KD Validated
mouse	WB	Cell Mol Immunol; BRD7 plays an anti-inflammatory role during early acute inflammation by inhibiting activation of the NF-кB signaling pathway.; Authors - Ran Zhao ; KO Validated
human	WB,IHC	Oncotarget; Bromodomain-containing protein 7 (BRD7) as a potential tumor suppressor in hepatocellular carcinoma.; Authors - Chang-Long Chen