Anticorps Polyclonal de lapin anti-BOP1

BOP1 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF

Conjugaison

Non conjugué

Publications

2

N° de cat : 28366-1-AP

Synonymes

block of proliferation 1, BOP1, KIAA0124

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Galerie de données de validation

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  WB analysis using 28366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28366-1-AP (BOP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 28366-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 28366-1-AP (BOP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 28366-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 28366-1-AP (BOP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 28366-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28366-1-AP (BOP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28366-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28366-1-AP (BOP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28366-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28366-1-AP (BOP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28366-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28366-1-AP (BOP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 28366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using 28366-1-AP (BOP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 28366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 28366-1-AP (BOP1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HeLa, cellules NCI-H1299
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-3, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

28366-1-AP cible BOP1 dans les applications de WB, IHC, IF et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BOP1 Protéine recombinante Ag26669
• Nom complet: block of proliferation 1
• Masse moléculaire calculée: 84 kDa
• Poids moléculaire observé: 117 kDa
• Numéro d’acquisition GenBank: BC013787
• Symbole du gène: BOP1
• Identification du gène (NCBI): 23246
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Block of Proliferation 1(BOP1) protein was first isolated from a mouse, and the truncation protein may led to cell cycle arrest. Bop1 plays a role in processing of the 28S and 5.8S rRNAs (ribosomal RNAs) to regulate ribosome biogenesis. And Bop1 also act as a component of Pes1/Bop1/WDR21 (PeBoW) complex for mature 60S ribosome, and consequently acts during cell division at G1 checkpoints (PMID:18670790; PMID:26940494). What's more, abnormal expression of BOP1 was associated with liver or colorectal cancers(PMID:16804918). The molecular weight of the protein we detected was slightly higher, about 117 kd, which is also seen in the article(PMID: 30100995).

Publications

Species	Application	Title
human	WB	Int J Biol Sci; Discovery of a novel HDACi structure that inhibits the proliferation of ovarian cancer cells in vivo and in vitro.; Authors - Miao Bai
human	WB	Front Oncol; Suppression of Ribosome Biogenesis by Targeting WD Repeat Domain 12 (WDR12) Inhibits Glioma Stem-Like Cell Growth.; Authors - Lanjuan Mi