Anticorps Polyclonal de lapin anti-BMP6

BMP6 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 55421-1-AP

Synonymes

BMP 6, BMP6, BMP-6, bone morphogenetic protein 6, VG 1 R, VG 1 related protein, VGR, VGR 1, VGR1

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Galerie de données de validation

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  WB analysis using 55421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55421-1-AP (BMP6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 55421-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55421-1-AP (BMP6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 55421-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 55421-1-AP (BMP6 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HL-60, cellules U2OS, tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

55421-1-AP cible BMP6 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: bone morphogenetic protein 6
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: NM_001718
• Symbole du gène: BMP6
• Identification du gène (NCBI): 654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Bone morphogenetic protein 6 (BMP6) is also named as VG-1-related protein (VG-1-R, VGR-1) and VGR, and belongs to the TGF-beta family. (PMID:33878365). Growth factor of the TGF-beta superfamily plays essential roles in many developmental processes including cartilage and bone formation (PMID:31019025). BMP6 participates in iron homeostasis, inhibits invasion by increasing adhesions and cell-cell type interactions and induces angiogenesis directly on vascular endothelial cells. BMP6 is coded by a tumor suppressor gene whose subexpression is related to the development and cancer progression; during neoplastic processes, methylation is the main mechanism by which gene silencing occurs (PMID:34706618). BMP6 plays an important role in the regulation of hepcidin/HAMP expression and iron metabolism by acting as a ligand for hemojuvelin/HJV (PMID:26582087). BMP6 initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2B (PMID:18070108).