Anticorps Monoclonal anti-BMI1

• Phare
• Validé par KD/KO

BMI1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, singe, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

CloneNo.

2E10A11

N° de cat : 66161-1-Ig

Synonymes

BMI1, PCGF4, Polycomb complex protein BMI 1, RING finger protein 51, RNF51

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Galerie de données de validation

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  WB analysis of PC-12 using 66161-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 66161-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 66161-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 66161-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 66161-1-Ig (BMI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66161-1-Ig

  IP Result of anti-BMI1 (IP:66161-1-Ig, 3ug; Detection:66161-1-Ig 1:1000) with HEK-293 cells lysate 1800ug.

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  IHC staining of human lymphoma using 66161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66161-1-Ig (BMI1 Antibody) at dilution of 1:50 under 40x lens.

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  IHC staining of human breast cancer using 66161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66161-1-Ig (BMI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 66161-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66161-1-Ig (BMI1 Antibody) at dilution of 1:50 under 10x lens.

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  IF Staining of COS-7 using 66161-1-Ig

  Immunofluorescent analysis of COS-7 cells using 66161-1-Ig (BMI1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules HT-1080, cellules U2OS, cellules U-937
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules COS-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

66161-1-Ig cible BMI1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, singe, souris

• Réactivité: Humain, singe, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BMI1 Protéine recombinante Ag21284
• Nom complet: BMI1 polycomb ring finger oncogene
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 37-43 kDa
• Numéro d’acquisition GenBank: BC011652
• Symbole du gène: BMI1
• Identification du gène (NCBI): 648
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.

Publications

Species	Application	Title
human	WB	Autophagy; Inhibition of BMI1 induces autophagy-mediated necroptosis.; Authors - Anindya Dey ; KD Validated
mouse	WB	Clin Transl Med; Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.; Authors - Haiyun Chen
mouse	WB	J Bone Miner Res; Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription; Authors - Qiuyi Wang
human	WB	Aging (Albany NY); Identification of a mesenchymal-related signature associated with clinical prognosis in glioma.; Authors - Zhengwei Zhang
human,mouse	WB,IHC	Mol Cancer Ther; Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.; Authors - Anindya Dey
human	WB,IF	Cancer Sci; Ubiquitin-specific protease 22 acts as an oncoprotein to maintain glioma malignancy through deubiquitinating B cell-specific Moloney murine leukemia virus integration site 1 for stabilization.; Authors - Guan-Zhong Qiu