Anticorps Polyclonal de lapin anti-BDNF

BDNF Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

111

Avis (1)

N° de cat : 28205-1-AP

Synonymes

Abrineurin, BDNF

---

Galerie de données de validation

• 

  WB analysis using 28205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28205-1-AP (BDNF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 28205-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 28205-1-AP (BDNF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 28205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28205-1-AP (BDNF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-12 using 28205-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using BDNF antibody (28205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules C6, cellules PC-12, cellules SH-SY5Y
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de souris, tissu de cervelet de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 97 publications below
• IHC: See 8 publications below
• IF: See 15 publications below

Informations sur le produit

28205-1-AP cible BDNF dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BDNF Protéine recombinante Ag28276
• Nom complet: brain-derived neurotrophic factor
• Masse moléculaire calculée: 247 aa, 28 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC029795
• Symbole du gène: BDNF
• Identification du gène (NCBI): 627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).

Publications

Species	Application	Title
		Autophagy; Trehalose induces autophagy via lysosomal-mediated TFEB activation in models of motoneuron degeneration.; Authors - Paola Rusmini
mouse	WB	Mater Today Bio; Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.; Authors - Luyu Wang
mouse	WB	ACS Appl Mater Interfaces; Reactive Oxygen Species Scavenging Functional Hydrogel Delivers Procyanidins for the Treatment of Traumatic Brain Injury in Mice.; Authors - Xuyang Huang
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
rat	WB	Neurobiol Stress; Low-dose S-ketamine exerts antidepressant-like effects via enhanced hippocampal synaptic plasticity in postpartum depression rats.; Authors - Zhuoyu Ren
mouse	WB	Brain Behav Immun; IL-33 in the basolateral amygdala integrates neuroinflammation into anxiogenic circuits via modulating BDNF expression.; Authors - Xiao Zhuang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Tanusree (Verified Customer) (12-18-2019)

Worked well in WB at 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain