Anticorps Monoclonal anti-BCL2

• Phare
• Validé par KD/KO

BCL2 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

4H8C6

N° de cat : CL555-60178

Synonymes

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, Oncogene B cell leukemia 2

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Galerie de données de validation

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  IF Staining of human appendicitis using CL555-60178

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of human appendicitis using CL555-60178

  Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of NIH/3T3 using CL555-60178

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  IF Staining of MCF-7 using CL555-60178

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®555 BCL2 antibody (CL555-60178, Clone: 4H8C6 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-60178

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human BCL2 (CL555-60178, Clone:4H8C6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: tissu d'appendicite humain, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-60178 cible BCL2 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BCL2 Protéine recombinante Ag3508
• Nom complet: B-cell CLL/lymphoma 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC027258
• Symbole du gène: BCL2
• Identification du gène (NCBI): 596
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

1) What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2) FAQs for Bcl-2

A) How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
B) What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.