Anticorps Monoclonal anti-BCAS3

BCAS3 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1G7B1

N° de cat : 67085-1-Ig

Synonymes

BCAS3, DKFZp686O1527, FLJ20128, GAOB1, MAAB, Protein Maab1

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Galerie de données de validation

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  WB analysis using 67085-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67085-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67085-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67085-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67085-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67085-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A431, cellules A549, cellules HEK-293, cellules Jurkat, cellules K-562, cellules LNCaP, tissu cérébral de porc, tissu cérébral de rat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67085-1-Ig cible BCAS3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BCAS3 Protéine recombinante Ag28545
• Nom complet: breast carcinoma amplified sequence 3
• Masse moléculaire calculée: 99 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC001250
• Symbole du gène: BCAS3
• Identification du gène (NCBI): 54828
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rudhira/BCAS3 (Breast Cancer Amplified Sequence 3) is a conserved protein expressed in the embryonic vasculature and malignant tumors. Rudhira is a predicted WD40 domain protein. During mouse development Rudhira is expressed in erythropoietic and angiogenic precursors but not in their differentiated progeny. This transient expression of Rudhira is also seen in normal adult angiogenesis. BCAS3, the human ortholog of Rudhira is 98% identical and the gene maps to a breakpoint of hematological neoplasms on chromosome 17q23.1. BCAS3 is also expressed in vascular development in embryoid bodies in vitro and was originally identified as a gene over-expressed in breast cancers. It is reported to be a target of metastasis associated protein-1 (MTA-1). Further BCAS3 expression is also upregulated in grade III and IV glioblastomas where it is misexpressed in the tumor cells.