Anticorps Monoclonal anti-BAG6

• Phare
• Validé par KD/KO

BAG6 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1B8D3

N° de cat : 66661-1-Ig

Synonymes

BAG 6, BAG6, BAT3, D6S52E, G3, HLA B associated transcript 3, Protein G3

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Galerie de données de validation

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  WB analysis using 66661-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of HEK-293 using 66661-1-Ig

  WB result of BAG6 antibody (66661-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG6 transfected HEK-293 cells.

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  WB analysis of HeLa using 66661-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66661-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66661-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66661-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66661-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66661-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66661-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3, cellules PC-12, cellules RAW 274.7
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66661-1-Ig cible BAG6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: BAG6 Protéine recombinante Ag24525
• Nom complet: HLA-B associated transcript 3
• Masse moléculaire calculée: 119 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC003133
• Symbole du gène: BAT3
• Identification du gène (NCBI): 7917
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.

Publications

Species	Application	Title
human	WB	Mol Cell; RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.; Authors - Xianyan Hu