Anticorps Polyclonal de lapin anti-BAG3

• Phare
• Validé par KD/KO

BAG3 Polyclonal Antibody for IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

N° de cat : CL594-10599

Synonymes

BAG 3, BAG3, BIS, CAIR 1, Docking protein CAIR 1

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Galerie de données de validation

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  IF Staining of A549 using CL594-10599

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 BAG3 antibody (CL594-10599) at dilution of 1:100.

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  IF Staining of A549 using CL594-10599

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 BAG3 antibody (CL594-10599) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF: cellules A549,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-10599 cible BAG3 dans les applications de IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BAG3 Protéine recombinante Ag0956
• Nom complet: BCL2-associated athanogene 3
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 74-80 kDa
• Numéro d’acquisition GenBank: BC006418
• Symbole du gène: BAG3
• Identification du gène (NCBI): 9531
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAG3 (Bcl2-associated athanogene 3) belongs to the BAG protein family, the co-chaperone that binds to Hsc70/Hsp70 through the BAG domain and modulates their activity in polypeptide folding. BAG3 contains also a WW domain and a proline-rich (PXXP) repeat, that mediate binding to partners different from Hsp70. Through interacting with different molecular partner, BAG3 influences several cell processes, such as apoptosis, autophagy and cell motility. BAG3 protein has been reported to sustain cell survival, resistance to therapy, and/or motility and metastatization in several tumor types, thus being identified as a potential target for anticancer therapies. In addition, defects in BAG3 are the cause of some myopathy. BAG3 normally migrates around 74-80 kDa; a slightly different molecular weight or a doublet form can be observed in some cell types and/or following cell exposure to stressors. A synaptosome associated form of 40 kDa has recently been described.