Anticorps Monoclonal anti-CD80/B7-1

CD80/B7-1 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

27

CloneNo.

1E2F10

N° de cat : 66406-1-Ig

Synonymes

Activation B7 1 antigen, B7, B7 1, B7-1, BB1, CD28LG, CD28LG1, CD80, CD80 molecule, CD80/B7-1, CTLA 4 counter receptor B7.1, LAB7

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Galerie de données de validation

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  WB analysis of DC2.4 using 66406-1-Ig

  DC2.4 cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80/B7-1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 66406-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 66406-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66406-1-Ig (CD80 / B7-1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 66406-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66406-1-Ig (CD80 / B7-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66406-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66406-1-Ig (CD80 / B7-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Daudi, cellules DC2.4
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 9 publications below
• IF: See 7 publications below
• FC: See 3 publications below

Informations sur le produit

66406-1-Ig cible CD80/B7-1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD80/B7-1 Protéine recombinante Ag5615
• Nom complet: CD80 molecule
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC042665
• Symbole du gène: CD80
• Identification du gène (NCBI): 941
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publications

Species	Application	Title
mouse	FC	Acta Pharm Sin B; Converting bacteria into autologous tumor vaccine via surface biomineralization of calcium carbonate for enhanced immunotherapy; Authors - Lina Guo
mouse	WB	ACS Appl Mater Interfaces; Surface Coating of Pulmonary siRNA Delivery Vectors Enabling Mucus Penetration, Cell Targeting, and Intracellular Radical Scavenging for Enhanced Acute Lung Injury Therapy.; Authors - Jiaojiao Zhu
human,mouse	IHC	Theranostics; ILT4 inhibition prevents TAM- and dysfunctional T cell-mediated immunosuppression and enhances the efficacy of anti-PD-L1 therapy in NSCLC with EGFR activation.; Authors - Xiaozheng Chen
mouse	WB	Free Radic Biol Med; Mutual antagonism between indoleamine 2,3-dioxygenase 1 and nuclear factor E2-related factor 2 regulates the maturation status of DCs in liver fibrosis.; Authors - Chan Mo
mouse	WB,IF	Life Sci; Activation of GABA receptor attenuates intestinal inflammation by modulating enteric glial cells function through inhibiting NF-κB pathway; Authors - Ziteng Deng
human	WB	Int J Biochem Cell Biol; Knockdown of LncRNA MALAT1 contributes to cell apoptosis via regulating NF-κB/CD80 axis in neonatal respiratory distress syndrome.; Authors - Chenxia Juan