• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anticorps Monoclonal anti-Annexin V

Annexin V Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1E6A8

N° de cat : CL647-66245

Synonymes

Anchorin CII, Annexin 5, annexin A5, Annexin V, ANX5, ANXA5, Calphobindin I, CBP I, Endonexin II, ENX2, Lipocortin V, PAP I, PP4, Thromboplastin inhibitor, VAC alpha, Vascular anticoagulant alpha

Galerie de données de validation

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 647-conjugated Annexin V Monoclonal (CL647-66245)

    IF Staining of HeLa using CL647-66245

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647 Annexin V antibody (CL647-66245, Clone: 1E6A8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated Annexin V Monoclonal (CL647-66245)

    FC experiment of HeLa using CL647-66245

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Annexin V (CL647-66245, Clone:1E6A8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Applications testées

Résultats positifs en IFcellules HeLa,
Résultats positifs en cytométriecellules HeLa

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-66245 cible Annexin V dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Annexin V Protéine recombinante Ag1538
Nom complet annexin A5
Masse moléculaire calculée 36 kDa
Numéro d’acquisition GenBankBC001429
Symbole du gène ANXA5
Identification du gène (NCBI) 308
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.

Protocole

Product Specific Protocols
IF protocol for CL Plus 647 Annexin V antibody CL647-66245Download protocol
Standard Protocols
Click here to view our Standard Protocols