Anticorps Polyclonal de lapin anti-AZI1

• Phare
• Validé par KD/KO

AZI1 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 25735-1-AP

Synonymes

5 azacytidine induced 1, AZ1, AZI1, Centrosomal protein of 131 kDa, Cep131, KIAA1118

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Galerie de données de validation

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  WB analysis of mouse testis using 25735-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25735-1-AP (AZI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 25735-1-AP

  Immunofluorescent analysis of HeLa cells using 25735-1-AP (AZI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

25735-1-AP cible AZI1 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AZI1 Protéine recombinante Ag22699
• Nom complet: 5-azacytidine induced 1
• Masse moléculaire calculée: 1083 aa, 122 kDa
• Poids moléculaire observé: 131 kDa
• Numéro d’acquisition GenBank: BC136578
• Symbole du gène: AZI1
• Identification du gène (NCBI): 22994
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.

Publications

Species	Application	Title
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
human	WB,IF,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li ; KD Validated
human,mouse	IF	Elife; Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis; Authors - Emma A Hall
human	IF	PLoS Biol; Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium.; Authors - Özge Z Aydin
human	WB	Front Oncol; USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.; Authors - Catherine M Nickson ; KD Validated
human	IF	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jay (Verified Customer) (10-15-2019)

Methanol fixation,Immunostaining with Azi1(red), centrosome marker gamma-tubulin(green) and DAPI(blue) for nucleus.First time to show IF result using mouse NIH3T3 cell line.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse NIH3T3

AZI1 Antibody Immunofluorescence, validation (1:100 dilution) in Mouse NIH3T3 (Cat no:25735-1-AP)