Anticorps Polyclonal de lapin anti-ATP8

ATP8 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

32

Avis (1)

N° de cat : 26723-1-AP

Synonymes

A6L, ATP synthase protein 8, ATP8, ATPASE8, F ATPase subunit 8, MT ATP8, MTATP8

---

Galerie de données de validation

• 

  WB analysis using 26723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26723-1-AP (ATP8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26723-1-AP (ATP8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 26723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26723-1-AP (ATP8 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 26723-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP8 antibody (26723-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules LNCaP, tissu cardiaque de rat
• Résultats positifs en IHC: tissu de cervelet de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 32 publications below

Informations sur le produit

26723-1-AP cible ATP8 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ATP synthase 8; ATPase subunit 8
• Masse moléculaire calculée: 8 kDa
• Poids moléculaire observé: 8 kDa
• Numéro d’acquisition GenBank: YP_003024030
• Symbole du gène: ATP8
• Identification du gène (NCBI): 4509
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP8 is a subunit of F0, the functional domain of mitochondrial ATP synthase or complex V. Mutation of ATP8 has been reported as the cause of apical hypertrophic cardiomyopathy and neuropathy.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing.; Authors - Li-Sheng Zhang
human	WB	Nucleic Acids Res; A coronary artery disease-associated tRNAThr mutation altered mitochondrial function, apoptosis and angiogenesis.; Authors - Zidong Jia
human	WB	Nucleic Acids Res; A deafness-associated tRNA mutation caused pleiotropic effects on the m1G37 modification, processing, stability and aminoacylation of tRNAIle and mitochondrial translation.; Authors - Feilong Meng
zebrafish	WB	Nucleic Acids Res; Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.; Authors - Qinghai Zhang
human	WB	Nucleic Acids Res; Hypertension-associated mitochondrial DNA 4401A>G mutation caused the aberrant processing of tRNAMet, all 8 tRNAs and ND6 mRNA in the light-strand transcript.; Authors - Xiaoxu Zhao
human	WB	J Cell Biol; Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.; Authors - Yumiko Oshima

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Mi (Verified Customer) (02-10-2023)

This antibody works very well in human adipocytes, a strong single band around 8 kd is detected when loading 10 ug total protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: adipocytes