Anticorps Monoclonal anti-ATP5A1

ATP5A1 Monoclonal Antibody for FC (Intra), IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, singe, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B10H3

N° de cat : CL488-66037

Synonymes

ATP5A, ATP5A1, ATP5AL2, ATPM, Cell and organelle markers, hATP1, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MOM2, OMR, ORM

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Galerie de données de validation

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  IF Staining of HepG2 using CL488-66037

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66037 (Green, ATP5A1 antibody) at dilution of 1:100 and CL594-66187 (Red, Cytokeratin 18 antibody) at dilution of 1:100.

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  IF Staining of HepG2 using CL488-66037

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66037 (ATP5A1 antibody) at dilution of 1:50.

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  IF Staining of HeLa using CL488-66037

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66037 (ATP5A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using CL488-66037

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ATP5A1 (CL488-66037, Clone:1B10H3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: cellules HepG2, cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66037 cible ATP5A1 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATP5A1 Protéine recombinante Ag8119
• Nom complet: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
• Masse moléculaire calculée: 60 kDa
• Numéro d’acquisition GenBank: BC064562
• Symbole du gène: ATP5A1
• Identification du gène (NCBI): 498
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a, b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues. The predicted MW of ATP5A1 is 60 kDa, while it undergoes the transit peptide cleavage to become a mature form around 50-55 kDa. Several isoforms of ATP5A1 exist due to the alternative splicing.

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C