Anticorps Monoclonal anti-ATG16L1

ATG16L1 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

1G5G7

N° de cat : 67943-1-Ig

Synonymes

APG16 like 1, APG16L, ATG16L, ATG16L1, Autophagy related protein 16 1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30

---

Galerie de données de validation

• 

  WB analysis using 67943-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis of rat lung using 67943-1-Ig

  rat lung tissue were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67943-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67943-1-Ig (ATG16L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 67943-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG16L1 antibody (67943-1-Ig, Clone: 1G5G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules 4T1, cellules HepG2, cellules NIH/3T3, cellules PC-12, tissu pulmonaire de rat
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

67943-1-Ig cible ATG16L1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ATG16L1 Protéine recombinante Ag14881
• Nom complet: ATG16 autophagy related 16-like 1 (S. cerevisiae)
• Masse moléculaire calculée: 607 aa, 68 kDa
• Poids moléculaire observé: 68-75 kDa
• Numéro d’acquisition GenBank: BC000061
• Symbole du gène: ATG16L1
• Identification du gène (NCBI): 55054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human ATG16L1 is a 607 amino acid protein (~68 kDa) comprising three major domains: the N‐terminal ATG5 binding domain (ATG5‐BD), the central coiled‐coil domain (CCD) and a predicted C‐terminal WD40‐domain. ATG16L1α and β (Atg16L1α, 63 kDa; and Atg16L1β, 71 kDa) are the major isoforms expressed in intestinal epithelium and macrophages , and all isoforms encode exon 9, which contains Thr 300. Atg16L1 mediates the cellular degradative process of autophagy and is considered a critical regulator of inflammation based on its genetic association with inflammatory bowel disease. ATG16L1 has been implicated in Crohn's disease. (PMID: 24553140, PMID: 22740627,PMID: 28685931)

Publications

Species	Application	Title
pig	WB	Front Immunol; Porcine cGAS-STING signaling induced autophagy inhibits STING downstream IFN and apoptosis; Authors - Nengwen Xia
human	WB	Epileptic Disord; lncRNA UCA1 induces autophagic gene expression via epigenetic regulation mediated by ATG16L1 and miR-132-3p in SH-SY5Y cells treated with retinoic acid; Authors - Fang Wen
human	WB	Mol Carcinog; Forkhead Box Q1 is a novel regulator of autophagy in breast cancer cells; Authors - Su-Hyeong Kim
human	WB,IF	Dev Cell; V-ATPase recruitment to ER exit sites switches COPII-mediated transport to lysosomal degradation; Authors - Yiwei Sun