Anticorps Polyclonal de lapin anti-ATG14/Barkor

ATG14/Barkor Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 28021-1-AP

Synonymes

ATG14, Atg14L, Barkor, KIAA0831

---

Galerie de données de validation

• 

  WB analysis using 28021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28021-1-AP (ATG14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 28021-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 28021-1-AP (ATG14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 28021-1-AP

  IP result of anti-ATG14(IP:28021-1-AP, 4ug; Detection:28021-1-AP 1:500) with rat brain tissue lysate 4000 ug.

• 

  IHC staining of human liver cancer using 28021-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28021-1-AP (ATG14 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28021-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28021-1-AP (ATG14 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de rat,
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below

Informations sur le produit

28021-1-AP cible ATG14/Barkor dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATG14/Barkor Protéine recombinante Ag27671
• Nom complet: KIAA0831
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 and 65 kDa
• Numéro d’acquisition GenBank: NM_014924
• Symbole du gène: KIAA0831
• Identification du gène (NCBI): 22863
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATG14 plays a critical role in autophagy which is required for both basal and inducible autophagy. It has been reported that the loss of ATG14 completely blocks autophagic fusion with lysosomes. ATG14 is associated with the location of PI3-kinase complex PI3KC3-C1 and the formation of autophagosome. Besides, BECN2 can form the BECN2:ATG14 heterodimer with ATG14. 28021-1-AP antibody recognizes the 55 kDa protein and the phosphorylated 65 kDa protein in SDS-PAGE. (PMID: 28218432, 24597603, 30894050)

Publications

Species	Application	Title
human	WB	Cancer Lett; Targeting PP2A with lomitapide suppresses colorectal tumorigenesis through the activation of AMPK/Beclin1-mediated autophagy.; Authors - Qian Zuo
human	WB	Ecotoxicol Environ Saf; Weakened interaction of ATG14 and the SNARE complex blocks autophagosome-lysosome fusion contributes to fluoride-induced developmental neurotoxicity.; Authors - Yuanli Zhang
mouse	WB	Cells; Cytosolic HMGB1 Mediates LPS-Induced Autophagy in Microglia by Interacting with NOD2 and Suppresses Its Proinflammatory Function; Authors - Seung-Woo Kim
human	WB	Life (Basel); MiR-371a-5p Positively Associates with Hepatocellular Carcinoma Malignancy but Sensitizes Cancer Cells to Oxaliplatin by Suppressing BECN1-Dependent Autophagy; Authors - Zhenbing Lv
mouse	WB	FASEB J; Melatonin ameliorates cerebral ischemia-reperfusion injury in diabetic mice by enhancing autophagy via the SIRT1-BMAL1 pathway; Authors - Lian Liu
human	WB,IHC	Heliyon; Elevated GABRP expression is correlated to the excessive autophagy in intrahepatic cholestasis of pregnancy; Authors - Ruihong Dong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Balawant (Verified Customer) (12-25-2023)

I have used this antibody, and it is working great. this antibody has specific western blot band and no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mice colon tissue and colon cancer cell line