Anticorps Monoclonal anti-ATF6
ATF6 Monoclonal Antibody for IF
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, rat, souris
Applications
IF
Conjugaison
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3B7E4
N° de cat : CL488-66563
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en IF | cellules HepG2, |
Dilution recommandée
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Informations sur le produit
CL488-66563 cible ATF6 dans les applications de IF et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Hôte / Isotype | Mouse / IgG1 |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | ATF6 Protéine recombinante Ag21456 |
Nom complet | activating transcription factor 6 |
Masse moléculaire calculée | 75 kDa |
Poids moléculaire observé | 90-100 kDa |
Numéro d’acquisition GenBank | BC014969 |
Symbole du gène | ATF6 |
Identification du gène (NCBI) | 22926 |
Conjugaison | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Forme | Liquide |
Méthode de purification | Purification par protéine G |
Tampon de stockage | PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3. |
Conditions de stockage | Stocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.
Protocole
Product Specific Protocols | |
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IF protocol for CL Plus 488 ATF6 antibody CL488-66563 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |