Anticorps Monoclonal anti-ATF4

ATF4 Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

FC (Intra)

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

3E4C5

N° de cat : FITC-60035

Synonymes

ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, TAXREB67, TXREB



Applications testées

Résultats positifs en cytométriecellules HepG2

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-60035 cible ATF4 dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène ATF4 Protéine recombinante Ag1279
Nom complet activating transcription factor 4 (tax-responsive enhancer element B67)
Masse moléculaire calculée 39 kDa
Poids moléculaire observé45-50 kDa
Numéro d’acquisition GenBankBC022088
Symbole du gène ATF4
Identification du gène (NCBI) 468
Conjugaison FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the molecular weight of ATF4?

The molecular weight of ATF is 38.6 kD.

 

What is ATF4?

Activating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor (PMIDs: 16000305, 17485283).

 

What the function of ATF4?

ATF4 its a transcription factor that controls the transcriptional activity of mature osteoblasts. ATF4 is particularly critical for their timely onset and terminal differentiation, as well as expression of Bsp and osteocalcin. Knockout animals displayed reduction or delay in bone mineralization and have severely reduced bone volume. ATF4 is also part of the PERK-eIF2α-ATF4-CHOP apoptosis pathway, which is activated by ER stress, and it likely plays a role related to tumor cell survival (PMIDs: 18083928, 16000305, 30134550).

 

What is the effect of ATF4 interaction with RSK2?

ATF4 and RSK2 posttranscriptionally regulate type I collagen synthesis. Lack of RSK2 phosphorylation of AFT4 may contribute to skeletal phenotypes associated with Coffin-Lowry Syndrome (PMID: 17485283).

 

Where is ATF4 expressed?

ATF4 protein is predominantly expressed in osteoblasts, although its corresponding Atf4 mRNA is ubiquitously expressed (PMID: 16000305).

 

What regulates ATF4 expression?

ATF4 is regulated by a ubiquitin/proteasomal pathway, which is less active in osteoblasts by inhibition with MG115 (PMID: 16000305).

 

How does ATF4 expression affect Ocn mRNA?

Inhibition of the degradation pathway leads to ATF4 accumulation and induces Ocn mRNA expression in non-osteoblastic cells (PMID: 16000305).

 

Does ATF4 have the ability to induce osteoblast-specific gene expression even in non-osteoblastic cells?

Yes, ATF4, as well as other osteoblast differentiation factors, has this ability. AFT4 interactions with Runx2 can stimulate osteoblast-specific osteocalcin gene expression. (PMIDs: 16000305, 17485283)


Protocole

Product Specific Protocols
FC protocol for FITC Plus ATF4 antibody FITC-60035Download protocol
Standard Protocols
Click here to view our Standard Protocols