Anticorps Monoclonal anti-ASL

ASL Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1G9H3

N° de cat : 67692-1-Ig

Synonymes

argininosuccinate lyase, Arginosuccinase, ASAL, ASL

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Galerie de données de validation

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  WB analysis using 67692-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67692-1-Ig (ASL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 67692-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67692-1-Ig (ASL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67692-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67692-1-Ig (ASL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu rénal de porc, cellules K-562, cellules LNCaP, tissu hépatique de porc, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67692-1-Ig cible ASL dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ASL Protéine recombinante Ag30517
• Nom complet: argininosuccinate lyase
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC008195
• Symbole du gène: ASL
• Identification du gène (NCBI): 435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Argininosuccinate lyase (ASL), one of the significant UC enzymes, catalyzes argininosuccinate cleavage to generate arginine and fumarate. Arginine is then catalyzed by arginase to ornithine and polyamines, which are found to promote cancer cell proliferation and growth. Importantly, ASL ectopic expression is closely associated with poor prognosis of colorectal cancer, hepatocellular. ASL is a member of the aspartase/fumarase superfamily. Enzymes of this superfamily share similar tetrameric structure and active site, though the sequence identities between different members are quite low (less than 20%). Members of this superfamily have been recognised as drug targets for microbial infections. ASL is a 464-amino-acid enzyme with a molecular mass of 49.7 kDa. (PMID: 22386318 PMID: 31018905)

Publications

Species	Application	Title
mouse	WB	EMBO Mol Med; A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy; Authors - Nneka Southwell