Anticorps Monoclonal anti-ASK1

ASK1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

CloneNo.

1C3A10

N° de cat : 67072-1-Ig

Synonymes

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5

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Galerie de données de validation

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  WB analysis of HSC-T6 using 67072-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67072-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67072-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67072-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67072-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67072-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67072-1-Ig (ASK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67072-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67072-1-Ig (ASK1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of RAW 264.7 using 67072-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using ASK1 antibody (67072-1-Ig, Clone: 1C3A10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu cardiaque de souris, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

67072-1-Ig cible ASK1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ASK1 Protéine recombinante Ag5818
• Nom complet: mitogen-activated protein kinase kinase kinase 5
• Masse moléculaire calculée: 155 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC054503
• Symbole du gène: MAP3K5
• Identification du gène (NCBI): 4217
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 67072-1-Ig antibody detects the degraded product of ASK1 in SDS-PAGE.

Publications

Species	Application	Title
human,rat	WB	Aging (Albany NY); Jian-Pi-Yi-Shen decoction inhibits mitochondria-dependent granulosa cell apoptosis in a rat model of POF; Authors - Xiao-Lin Jiang
rat	WB	Front Endocrinol (Lausanne); Cangfudaotan decoction inhibits mitochondria-dependent apoptosis of granulosa cells in rats with polycystic ovarian syndrome; Authors - Xiao-Lin Jiang
human	WB	Cells; Orphan Nuclear Receptor Nur77 Mediates the Lethal Endoplasmic Reticulum Stress and Therapeutic Efficacy of Cryptomeridiol in Hepatocellular Carcinoma; Authors - Xudan Li
rat	IF	Mol Med Rep; ASK1 inhibitor NQDI‑1 decreases oxidative stress and neuroapoptosis via the ASK1/p38 and JNK signaling pathway in early brain injury after subarachnoid hemorrhage in rats; Authors - Jiajia Duan
rat	WB,IF	Phytomedicine; Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology; Authors - Weina Qian
mouse		Front Pharmacol; Erratum: Addendum: A novel N-Arylpyridone compound alleviates the inflammatory and fibrotic reaction of silicosis by inhibiting the ASK1-p38 pathway and regulating macrophage polarization; Authors - Mingming Fan