Anticorps Polyclonal de lapin anti-ASIC1

• Phare
• Validé par KD/KO

ASIC1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 27235-1-AP

Synonymes

ACCN2, Acid sensing ion channel 1, ASIC, ASIC1, ASIC1A, BNaC2, Brain sodium channel 2, hBNaC2

---

Galerie de données de validation

• 

  WB analysis using 27235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27235-1-AP (ASIC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 27235-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27235-1-AP (ASIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 27235-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27235-1-AP (ASIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 27235-1-AP

  IP Result of anti-ASIC1 (IP:27235-1-AP, 4ug; Detection:27235-1-AP 1:300) with mouse brain tissue lysate 3000ug.

• 

  IHC staining of human pancreas cancer using 27235-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27235-1-AP (ASIC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 27235-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 27235-1-AP (ASIC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27235-1-AP (ASIC1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27235-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27235-1-AP (ASIC1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de cervelet de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

27235-1-AP cible ASIC1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASIC1 Protéine recombinante Ag25941
• Nom complet: amiloride-sensitive cation channel 2, neuronal
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 60-70 kDa, 80-90 kDa
• Numéro d’acquisition GenBank: NM_001095
• Symbole du gène: ACCN2
• Identification du gène (NCBI): 41
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ASIC1(Acid-sensing ion channel 1), also named as ACCN2, BNaC2 or amiloride-sensitive cation channel 2, is a member of the ASICs family. ASICs, an H+-gated subgroup of the epithelial Na+ channel/degenerin (ENaC/DEG) superfamily, are widely expressed throughout the central and peripheral nervous system and triggered by increased extracellular acidification (PMID: 28518134, 27941930). ASIC1 is a key player in acidosis-induced tumor growth and invasiveness. Clinically, alterations of ASIC1 are associated with poor survival in breast cancer (PMID: 26686084).

Publications

Species	Application	Title
mouse	WB,IF	Research (Wash D C); Targeting ASIC1a Promotes Neural Progenitor Cell Migration and Neurogenesis in Ischemic Stroke; Authors - Hongfei Ge
rat	WB	Cell Signal; Paeoniflorin inhibits pyroptosis of nucleus pulposus cells in an acidic environment and alleviates the degeneration of the intervertebral disc in rats; Authors - Wang-Ying Dai
human	IHC,FC	Lab Invest; Acidosis induces synovial fibroblasts to release vascular endothelial growth factor via acid-sensitive ion channel 1a.; Authors - Xuewen Qian
mouse	FC	Lab Invest; ASIC1a promotes the proliferation of synovial fibroblasts via the ERK/MAPK pathway.; Authors - Jingjing Tao
mouse	WB	Eur J Pharmacol; The interaction of ASIC1a and ERS mediates nerve cell apoptosis induced by insulin deficiency.; Authors - Xuesheng Pan ; KD Validated
human,mouse	WB,IF,CoIP	J Adv Res; ASIC1/RIP1 accelerates atherosclerosis via disrupting lipophagy; Authors - Yuan-Mei Wang