Anticorps Monoclonal anti-ASAH1

• Phare
• Validé par KD/KO

ASAH1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1B7E4

Avis (1)

N° de cat : 67092-1-Ig

Synonymes

AC, Acid ceramidase, Acylsphingosine deacylase, ASAH, ASAH1, FLJ21558, FLJ22079, HSD 33, PHP, PHP32, Putative 32 kDa heart protein

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Galerie de données de validation

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  WB analysis of rat cerebellum using 67092-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67092-1-Ig (ASAH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 67092-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67092-1-Ig (ASAH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67092-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67092-1-Ig (ASAH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cerebellum using 67092-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67092-1-Ig (ASAH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67092-1-Ig (ASAH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67092-1-Ig (ASAH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 67092-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 67092-1-Ig (ASAH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de rat, tissu cérébral de rat, tissu cérébral de souris, tissu de cervelet de porc
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

67092-1-Ig cible ASAH1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ASAH1 Protéine recombinante Ag28645
• Nom complet: N-acylsphingosine amidohydrolase (acid ceramidase) 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC016828
• Symbole du gène: ASAH1
• Identification du gène (NCBI): 427
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.

Publications

Species	Application	Title
mouse	WB,IF,IP	Mol Med; Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis; Authors - Juan Xiao ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (01-20-2023)

Good antibody. May not be able to use on certain mouse tissues.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Mouse brain

ASAH1 Antibody Western Blot validation (1:4000 dilution) in Mouse brain (Cat no:67092-1-Ig)