Anticorps Monoclonal anti-RhoGDI

• Phare
• Validé par KD/KO

RhoGDI Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1B1G9

N° de cat : 66480-1-Ig

Synonymes

aGDI, ARHGDIA, GDIA1, Rho GDI 1, Rho GDI alpha, RHOGDI, RHOGDI 1

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Galerie de données de validation

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  WB analysis using 66480-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66480-1-Ig (ARHGDIA,aGDI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66480-1-Ig

  WB result of RhoGDI antibody (66480-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RhoGDI transfected HEK-293 cells.

• 

  WB analysis using 66480-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66480-1-Ig (ARHGDIA,aGDI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HEK-293, cellules Jurkat, cellules NIH/3T3, cellules RAW 264.7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66480-1-Ig cible RhoGDI dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RhoGDI Protéine recombinante Ag0791
• Nom complet: Rho GDP dissociation inhibitor (GDI) alpha
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC005851
• Symbole du gène: ARHGDIA
• Identification du gène (NCBI): 396
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.