Anticorps Polyclonal de lapin anti-ARHGAP10

• Phare
• Validé par KD/KO

ARHGAP10 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 55139-1-AP

Synonymes

ARHGAP10, Graf related protein 2, GRAF2, PS GAP, PSGAP

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Galerie de données de validation

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  WB analysis of mouse heart using 55139-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 55139-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 55139-1-AP (ARHGAP10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 55139-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55139-1-AP (ARHGAP10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55139-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55139-1-AP (ARHGAP10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55139-1-AP (ARHGAP10 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55139-1-AP (ARHGAP10 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 55139-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 55139-1-AP (ARHGAP10 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HEK-293, tissu cardiaque humain, tissu rénal de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below

Informations sur le produit

55139-1-AP cible ARHGAP10 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Rho GTPase activating protein 10
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 89 kDa
• Numéro d’acquisition GenBank: NM_024605
• Symbole du gène: ARHGAP10
• Identification du gène (NCBI): 79658
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ARHGAP10, also named as GRAF2, is GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. It is essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. ARHGAP10 inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. It stabilizes PAK-2p34 thereby increasing stimulation of cell death. The antibody is specific to ARHGAP10.

Publications

Species	Application	Title
human	WB	Nat Metab; Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression; Authors - Lin Qian
human	WB	Mol Ther Nucleic Acids; miR-3174 Contributes to Apoptosis and Autophagic Cell Death Defects in Gastric Cancer Cells by Targeting ARHGAP10.; Authors - Bowen Li ; KD Validated
mouse	WB	Front Cell Dev Biol; The Beta-Tubulin Isotype TUBB6 Controls Microtubule and Actin Dynamics in Osteoclasts.; Authors - Justine Maurin
human	IHC	Front Oncol; Identification and Validation of an Apoptosis-Related Gene Prognostic Signature for Oral Squamous Cell Carcinoma.; Authors - Shuqin Wang
mouse	WB	J Biol Chem; The Cdc42 Guanine Nucleotide Exchange Factor FGD6 Coordinates Cell Polarity and Endosomal Membrane Recycling in Osteoclasts.; Authors - Charlotte Steenblock
mouse	WB	J Neurol; Rho GTPase-activating protein 10 (ARHGAP10/GRAF2) is a novel autoantibody target in patients with autoimmune encephalitis; Authors - Sven Jarius