Anticorps Monoclonal anti-Arginase-1

Arginase-1 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

5D6D12

N° de cat : CL594-66129

Synonymes

ARG1, Arginase 1, arginase, liver, Arginase-1, Liver type arginase, Type I arginase



Applications testées

Résultats positifs en IFcellules HepG2,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

IFSee 1 publications below

Informations sur le produit

CL594-66129 cible Arginase-1 dans les applications de IF et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Réactivité citéeHumain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Arginase-1 Protéine recombinante Ag8810
Nom complet arginase, liver
Masse moléculaire calculée236aa,25 kDa; 322aa,35 kDa
Numéro d’acquisition GenBankBC005321
Symbole du gène ARG1
Identification du gène (NCBI) 383
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation(PMID:12098359) and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539).It can exsit as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing.Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control.(PMID:27117406). ARG1 is a novel biomarker of post-stroke immune suppression(2016). The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocole

Product Specific Protocols
IF protocol for CL594 Arginase-1 antibody CL594-66129Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Adv Sci (Weinh)

Wireless Localized Electrical Stimulation Generated by an Ultrasound-Driven Piezoelectric Discharge Regulates Proinflammatory Macrophage Polarization.

Authors - Ying Kong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Rachel (Verified Customer) (08-09-2022)

This ab works really well for IF in murine liver samples (formalin-fixed).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse liver