Anticorps Monoclonal anti-APOE

APOE Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

CloneNo.

1B2C9

Avis (1)

N° de cat : 66830-1-Ig

Synonymes

AD2, Apo E, APOE, apolipoprotein E, LPG

---

Galerie de données de validation

• 

  WB analysis of human plasma using 66830-1-Ig

  human plasma was subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at a range of dilutions from 1:5000 to 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66830-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66830-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:3400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 66830-1-Ig

  human blood was subjected to SDS PAGE followed by western blot with 66830-1-Ig (APOE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 66830-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66830-1-Ig (APOE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66830-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOE antibody (66830-1-Ig, Clone: 1B2C9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 66830-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APOE (66830-1-Ig, Clone:1B2C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: tissu plasmatique humain, cellules Caco-2, cellules T-47D, plasma humain, sang humain, Sanguin humain
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:20000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

66830-1-Ig cible APOE dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: APOE Protéine recombinante Ag28186
• Nom complet: apolipoprotein E
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 34-36 kDa
• Numéro d’acquisition GenBank: BC003557
• Symbole du gène: APOE
• Identification du gène (NCBI): 348
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

Publications

Species	Application	Title
human	WB,IF	Elife; A critical role of VMP1 in lipoprotein secretion.; Authors - Hideaki Morishita
mouse	IF	J Biol Chem; Lack of ApoE inhibits ADan amyloidosis in a mouse model of Familial Danish Dementia; Authors - Anllely Fernandez
mouse	WB	Front Immunol; Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.; Authors - Anika Alberts
human	IHC,IF	Mol Oncol; Single-cell and spatial analyses reveal the association between gene expression of glutamine synthetase with the immunosuppressive phenotype of APOE+CTSZ+TAM in cancers; Authors - Jinfen Wei
mouse	WB,IF	Nat Neurosci; Lipid-accumulated reactive astrocytes promote disease progression in epilepsy; Authors - Zhang-Peng Chen
human	WB	Cancer Sci; Discovery of lipid profiles in plasma-derived extracellular vesicles as biomarkers for breast cancer diagnosis; Authors - Lin Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Silvia (Verified Customer) (12-03-2019)

Specific antibody for IF and WB on Human tissues

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: WB 1:3000, IF 1:500
• Cell Tissue Type: Human organoids

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C