Anticorps Monoclonal anti-APOBEC3G

• Phare
• Validé par KD/KO

APOBEC3G Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF

Conjugaison

Non conjugué

Publications

2

CloneNo.

5G10A8

N° de cat : 60100-1-Ig

Synonymes

APOBEC related protein, APOBEC related protein 9, APOBEC3G, ARCD, ARP 9, ARP9, bK150C2.7, CEM 15, CEM15, MDS019

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Galerie de données de validation

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  WB analysis of PC-3 cells using 60100-1-Ig

  WB result of APOBEC3G antibody (60100-1-Ig, 1:1000) with si-Control and si-APOBEC3G transfected PC-3 cells.

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  WB analysis of A375 using 60100-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 60100-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 60100-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 60100-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 60100-1-Ig (APOBEC3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 60100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 60100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 60100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 60100-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60100-1-Ig(APOBEC3G antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of C6 using 60100-1-Ig

  Immunofluorescent analysis of C6 cells using 60100-1-Ig (APOBEC3G antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Applications testées

• Résultats positifs en WB: cellules A375, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules C6

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

60100-1-Ig cible APOBEC3G dans les applications de WB, IHC, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: APOBEC3G Protéine recombinante Ag7770
• Nom complet: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC024268
• Symbole du gène: APOBEC3G
• Identification du gène (NCBI): 60489
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Sci Rep; Hepatitis B virus X protein is capable of down-regulating protein level of host antiviral protein APOBEC3G.; Authors - Ruidong Chen
human	IHC	Int J Clin Exp Med; APOBEC3G expression is correlated with poor prognosis in colon carcinoma patients with hepatic metastasis.; Authors - Huanrong Lan