Anticorps Monoclonal anti-Apolipoprotein AI

Apolipoprotein AI Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

8

CloneNo.

1C9G5

N° de cat : 66206-1-Ig

Synonymes

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1

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Galerie de données de validation

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  WB analysis of human plasma using 66206-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66206-1-Ig (APOA1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66206-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66206-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 66206-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 66206-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66206-1-Ig (APOA1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66206-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66206-1-Ig (APOA1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66206-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Apolipoprotein AI (66206-1-Ig, Clone:1C9G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below

Informations sur le produit

66206-1-Ig cible Apolipoprotein AI dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Apolipoprotein AI Protéine recombinante Ag21920
• Nom complet: apolipoprotein A-I
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 26-28 kDa
• Numéro d’acquisition GenBank: BC005380
• Symbole du gène: APOA1
• Identification du gène (NCBI): 335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Publications

Species	Application	Title
human	WB	Elife; A critical role of VMP1 in lipoprotein secretion.; Authors - Hideaki Morishita
mouse	WB	Mol Cell Proteomics; Differential proteomic analysis of gender-dependent hepatic tumorigenesis in Hras12V transgenic mice.; Authors - Zhuona Rong
human,rat	WB	Peptides; Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor.; Authors - Bingyang Liu
mouse	WB	JCI Insight; Aldehyde dehydrogenase 2 and PARP1 interaction modulates hepatic HDL biogenesis by LXRα-mediated ABCA1 expression.; Authors - Luxiao Li
mouse	WB	Front Nutr; Fenofibrate enhances lipid deposition via modulating PPARγ, SREBP-1c, and gut microbiota in ob/ob mice fed a high-fat diet; Authors - Ying Zhang
human,mouse	WB	Int J Mol Sci; Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes; Authors - Zhonghao Li

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C