Anticorps Monoclonal anti-Annexin A2

• Phare
• Validé par KD/KO

Annexin A2 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

15

CloneNo.

1C1E12

N° de cat : 66035-1-Ig

Synonymes

Annexin 2, annexin A2, Annexin II, ANX2, ANX2L4, ANXA2, CAL1H, Calpactin 1 heavy chain, Calpactin I heavy chain, Chromobindin 8, LIP2, Lipocortin II, LPC2, LPC2D, P36, PAP IV, Protein I

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Galerie de données de validation

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  WB analysis using 66035-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66035-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66035-1-Ig

  WB result of Annexin A2 antibody (66035-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A2 transfected HEK-293 cells.

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  WB analysis using 66035-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66035-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66035-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66035-1-Ig (ANXA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66035-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66035-1-Ig (ANXA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66035-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66035-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 66035-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 66035-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66035-1-Ig (Annexin A2 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of T-47D using 66035-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 66035-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66035-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66035-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of K-562 using 66035-1-Ig

  1X10^6 K-562 cells were stained with 0.2ug Annexin A2 antibody (66035-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules 4T1, cellules A431, cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules MCF-7, cellules NIH/3T3, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules T-47D, cellules HeLa
• Résultats positifs en cytométrie: cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF): IF : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 9 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below

Informations sur le produit

66035-1-Ig cible Annexin A2 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Annexin A2 Protéine recombinante Ag16654
• Nom complet: annexin A2
• Masse moléculaire calculée: 339 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC016774
• Symbole du gène: ANXA2
• Identification du gène (NCBI): 302
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

Publications

Species	Application	Title
human	WB,IHC,IF,IP	Oncogene; Hypoxia-induced circADAMTS6 in a TDP43-dependent manner accelerates glioblastoma progression via ANXA2/ NF-κB pathway; Authors - Shulin Zhao ; KD Validated
human		Pharmacol Res; Chip-DSF: A rapid screening strategy for drug protein targets.; Authors - Zhao Cui
human	WB, IF, IP	J Exp Clin Cancer Res; Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma.; Authors - Lu Deng
human,mouse	WB,IHC,IF,CoIP	Biomed Pharmacother; Chlorogenic acid inhibits the proliferation of human lung cancer A549 cell lines by targeting annexin A2 in vitro and in vivo.; Authors - Lei Wang
human	WB	Biosci Rep; Role of Annexin A2 isoform2 on the aggregative growth of dermal papillae cells.; Authors - Jing Gu ; KD Validated
		Analyst; An iTRAQ based quantitative proteomic strategy to explore novel secreted proteins in metastatic hepatocellular carcinoma cell lines.; Authors - Yu Yanyan Y