Anticorps Monoclonal anti-ANGPTL4

ANGPTL4 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1D2H6

N° de cat : 67577-1-Ig

Synonymes

angiopoietin like 4, Angiopoietin like protein 4, Angiopoietin related protein 4, ANGPTL4, ARP4, FIAF, HFARP, NL2, PGAR, pp1158, PSEC0166, UNQ171/PRO197

---

Galerie de données de validation

• 

  WB analysis of human placenta using 67577-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67577-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67577-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 67577-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 67577-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67577-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67577-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67577-1-Ig (ANGPTL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of NIH/3T3 using 67577-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using ANGPTL4 antibody (67577-1-Ig, Clone: 1D2H6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules A549, cellules Caco-2, cellules COLO 320, cellules HT-29, tissu cérébral de souris
• Résultats positifs en IF: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67577-1-Ig cible ANGPTL4 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ANGPTL4 Protéine recombinante Ag30070
• Nom complet: angiopoietin-like 4
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC023647
• Symbole du gène: ANGPTL4
• Identification du gène (NCBI): 51129
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three isoforms, 45 kDa, 41 kDa and 27 kDa.

Publications

Species	Application	Title
human	WB,IF	BMC Biol; Molecular, cellular and neurological consequences of infection by the neglected human pathogen Nocardia; Authors - Xingzhao Ji