Anticorps Monoclonal anti-AMPK Alpha 1

• Phare
• Validé par KD/KO

AMPK Alpha 1 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

60

CloneNo.

1H8E1

N° de cat : 66536-1-Ig

Synonymes

AMPK, AMPK alpha, AMPK Alpha 1, AMPK subunit alpha 1, AMPK1, AMPKa1, PRKAA1

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Galerie de données de validation

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  WB analysis of HEK-293 using 66536-1-Ig

  WB result of AMPK alpha antibody (66536-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK alpha transfected HEK-293 cells.

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  WB analysis using 66536-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66536-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66536-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66536-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66536-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66536-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66536-1-Ig (AMPK alpha antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66536-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66536-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66536-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66536-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66536-1-Ig (AMPK alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules 4T1, cellules A549, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules HT-29, cellules Jurkat, cellules K-562, cellules L02, cellules MCF-7, cellules NIH/3T3, cellules PC-12, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 58 publications below
• IHC: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

66536-1-Ig cible AMPK Alpha 1 dans les applications de WB, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, porc, souris, Hamster
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AMPK Alpha 1 Protéine recombinante Ag24744
• Nom complet: protein kinase, AMP-activated, alpha 1 catalytic subunit
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC012622
• Symbole du gène: PRKAA1
• Identification du gène (NCBI): 5562
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Redox Biol; Adiponectin/AdiopR1 signaling prevents mitochondrial dysfunction and oxidative injury after traumatic brain injury in a SIRT3 dependent manner.; Authors - Shenghao Zhang
human,hamster	WB	Biomed Pharmacother; Berberine is a potential alternative for metformin with good regulatory effect on lipids in treating metabolic diseases; Authors - Hui-Hui Guo
mouse	WB,IHC	J Nanobiotechnology; Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles.; Authors - Chunai Gong
human	WB	J Nanobiotechnology; Graphene oxide exacerbates dextran sodium sulfate-induced colitis via ROS/AMPK/p53 signaling to mediate apoptosis.; Authors - Siliang Liu
human	WB,FC	Food Chem; Selective transportation and energy homeostasis regulation of dietary advanced glycation end-products in human intestinal Caco-2 cells.; Authors - Yi Wu
human	WB	Int J Mol Sci; Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells.; Authors - Weiyun Zhang