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Anticorps Monoclonal anti-ALDH1A1

ALDH1A1 Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

IF

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1A10A2

N° de cat : CL594-60171

Synonymes

ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated ALDH1A1 Monoclonal antibod (CL594-60171)

    IF Staining of HeLa using CL594-60171

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 ALDH1A1 antibody (CL594-60171, Clone: 1A10A2 ) at dilution of 1:100.

Applications testées

Résultats positifs en IFcellules HeLa,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-60171 cible ALDH1A1 dans les applications de IF et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène ALDH1A1 Protéine recombinante Ag8551
Nom complet aldehyde dehydrogenase 1 family, member A1
Masse moléculaire calculée 501 aa, 55 kDa
Numéro d’acquisition GenBankBC001505
Symbole du gène ALDH1A1
Identification du gène (NCBI) 216
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Protocole

Product Specific Protocols
IF protocol for CL594 ALDH1A1 antibody CL594-60171Download protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Vanessa (Verified Customer) (10-12-2023)

Tried many times different concentrations (until 1:50) and no signal.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Brain