Anticorps Monoclonal anti-ALDH1A1

• Phare
• Validé par KD/KO

ALDH1A1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

9

CloneNo.

1A10A2

N° de cat : 60171-1-Ig

Synonymes

ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1

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Galerie de données de validation

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  WB analysis of K-562 using 60171-1-Ig

  WB result of ALDH1A1 antibody (60171-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH1A1 transfected K-562 cells.

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  WB analysis using 60171-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of MCF-7 using 60171-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 60171-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 60171-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 60171-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60171-1-Ig (ALDH1A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human brain using 60171-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60171-1-Ig (ALDH1A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of mouse colon using 60171-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 60171-1-Ig (ALDH1A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 60171-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDH1A1 antibody (60171-1-Ig, Clone: 1A10A2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse colon using 60171-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 60171-1-Ig (ALDH1A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of A431 using 60171-1-Ig

  Immunofluorescent analysis of A431 cells using 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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  FC experiment of HepG2 using 60171-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ALDH1A1 (60171-1-Ig, Clone:1A10A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2, cellules HuH-7, cellules K-562, cellules MCF-7, tissu hépatique de porc, tissu hépatique de rat
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules A431, tissu de côlon de souris
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

60171-1-Ig cible ALDH1A1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ALDH1A1 Protéine recombinante Ag8551
• Nom complet: aldehyde dehydrogenase 1 family, member A1
• Masse moléculaire calculée: 501 aa, 55 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC001505
• Symbole du gène: ALDH1A1
• Identification du gène (NCBI): 216
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).

Publications

Species	Application	Title
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou
human	WB,CoIP	iScience; Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression; Authors - Zhilei Zhang ; KD Validated
human	WB	Front Pharmacol; Autophagy Inhibition Enhances the Anti-Tumor Activity of Methylseleninic Acid in Cisplatin-Resistance Human Lung Adenocarcinoma Cells.; Authors - Ming Xin
human	WB	Oral Dis; microRNA-17 is a tumor suppressor in oral squamous cell carcinoma and is repressed by LSD1.; Authors - Kaixin Wangzhou
human	WB	Mol Med Rep; lncRNA TPTEP1 inhibits stemness and radioresistance of glioma through miR‑106a‑5p‑mediated P38 MAPK signaling.; Authors - Ting Tang
human	WB	Cancer Cell Int; PMID: 31139022; Authors - Rui-Qi Wang

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C