Anticorps Polyclonal de lapin anti-AKT

AKT Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (2)

N° de cat : 55230-1-AP

Synonymes

AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

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Galerie de données de validation

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  WB analysis of HeLa using 55230-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55230-1-AP (AKT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 55230-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55230-1-AP (AKT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 55230-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55230-1-AP (AKT1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 55230-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55230-1-AP (AKT1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human prostate cancer using 55230-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55230-1-AP (AKT1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 55230-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55230-1-AP (AKT1 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

55230-1-AP cible AKT dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-akt murine thymoma viral oncogene homolog 1
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56-62 kDa
• Numéro d’acquisition GenBank: NM_001014431
• Symbole du gène: AKT1
• Identification du gène (NCBI): 207
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AKT1, also named as PKB and RAC, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and RAC subfamily. It plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. AKT1 promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. It plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. AKT1 mediates the antiapoptotic effects of IGF-I. (PMID:16139227)This antibody is specific to AKT1. It has no cross reaction to AKT2 and AKT3.

Publications

Species	Application	Title
human	WB	Mol Med Rep; Matrine inhibits the invasive and migratory properties of human hepatocellular carcinoma by regulating epithelial‑mesenchymal transition.; Authors - Yuwen Wang
mouse	WB	J Immunol; The Intracellular Interaction of Porcine β-Defensin 2 with VASH1 Alleviates Inflammation via Akt Signaling Pathway.; Authors - Lu Lu Li
mouse	WB	Int J Mol Sci; Extracellular Calcium-Induced Calcium Transient Regulating the Proliferation of Osteoblasts through Glycolysis Metabolism Pathways; Authors - Xiaohang Gao
rat	WB	J Ethnopharmacol; Shenqu xiaoshi oral solution enhances digestive function and stabilizes the gastrointestinal microbiota of juvenile rats with infantile anorexia; Authors - Meng Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution and 1:100 in IF

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Tanusree (Verified Customer) (08-14-2019)

The antibody works good in mouse brain tissue in western blotting.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain