Anticorps Monoclonal anti-AKT

• Phare
• Validé par KD/KO

AKT Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

329

CloneNo.

2C5D1

Avis (1)

N° de cat : 60203-2-Ig

Synonymes

AKT, AKT (pan), AKT pan, AKT1, AKT1/2/3, pan AKT, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha, total AKT

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Galerie de données de validation

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  WB analysis of A549 using 60203-2-Ig

  WB result of AKT antibody (60203-2-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected A549 cells.

• 

  WB analysis using 60203-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60203-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) and 66444-1-Ig(AKT1-phospho-S473 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60203-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60203-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60203-2-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 60203-2-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 60203-2-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 60203-2-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60203-2-Ig (AKT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 60203-2-Ig

  IP Result of anti-AKT (IP:60203-2-Ig, 5ug; Detection:60203-2-Ig 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-2-Ig (AKT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60203-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60203-2-Ig(AKT antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 60203-2-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using AKT antibody (60203-2-Ig, Clone: 2C5D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 60203-2-Ig

  Immunofluorescent analysis of HeLa cells using 60203-2-Ig (AKT antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

• 

  FC experiment of Jurkat using 60203-2-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human AKT (60203-2-Ig, Clone:2C5D1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules LNCaP, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7, cellules ROS1728, tissu cérébral de souris, tissu hépatique de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules MCF-7
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 321 publications below
• IHC: See 6 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

60203-2-Ig cible AKT dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, poulet, souris, Hamster
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AKT Protéine recombinante Ag16695
• Nom complet: v-akt murine thymoma viral oncogene homolog 1
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56-62 kDa
• Numéro d’acquisition GenBank: BC000479
• Symbole du gène: AKT1
• Identification du gène (NCBI): 207
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.

Publications

Species	Application	Title
mouse	WB	Cell Rep Med; Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing; Authors - Dongming Lv
human	WB	Adv Healthc Mater; A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.; Authors - Zhi Zheng
mouse	WB	Research (Wash D C); Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis; Authors - Lang Linghu
mouse,rat	WB	Toxicology; Protective role of cezanne in doxorubicin-induced cardiotoxicity by inhibiting autophagy, apoptosis and oxidative stress; Authors - Jiayan Zhang
rat	WB	Acta Biomater; Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.; Authors - Shanshan Ma
	WB	Sci Total Environ; Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario; Authors - Fangnon Firmin Fangninou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Janine (Verified Customer) (02-18-2019)

Yielded clear and distinguishable bands at the expected size of ~60kDa after 10 sec exposure using enhanced chemiluminescence.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: RPE-1 cell lysates (6cm dish)

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C