Anticorps Monoclonal anti-AKR1B1

• Phare
• Validé par KD/KO

AKR1B1 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

2F11G5

N° de cat : 67498-1-Ig

Synonymes

ADR, AKR1B1, Aldehyde reductase, Aldose reductase, ALDR1, ALR2, AR

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Galerie de données de validation

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  WB analysis of HeLa using 67498-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67498-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67498-1-Ig

  WB result of AKR1B1 antibody (67498-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR1B1 transfected HepG2 cells.

• 

  WB analysis of 4T1 using 67498-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67498-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67498-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67498-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67498-1-Ig (AKR1B1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules 4T1, cellules HeLa, cellules HepG2, cellules Jurkat, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

67498-1-Ig cible AKR1B1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AKR1B1 Protéine recombinante Ag7571
• Nom complet: aldo-keto reductase family 1, member B1 (aldose reductase)
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 34 kDa
• Numéro d’acquisition GenBank: BC000260
• Symbole du gène: AKR1B1
• Identification du gène (NCBI): 231
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; An Advanced Systems Pharmacology Strategy Reveals AKR1B1, MMP2, PTGER3 as Key Genes in the Competing Endogenous RNA Network of Compound Kushen Injection Treating Gastric Carcinoma by Integrated Bioinformatics and Experimental Verification; Authors - Wei Zhou
mouse	WB	Pharmacol Res; Inulin-type fructans change the gut microbiota and prevent the development of diabetic nephropathy.; Authors - Liman Luo